​​​Welcome to: FYST23 Experimental Biophysics, 15 credits​

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​​Welcome to the homepage of the course " Experimental biophysics". The aim of the course The course gives a specialisation in interdisciplinary work with a focus on experimental methods within biophysics. The course aims specifically at giving an introduction to the intersection of modern physics, nanotechnology, biomolecular chemistry and biology. By being based on current scientific articles, the course prepares the students for future research work.


Contents

  • The course contains three main parts. The first part of the course consists of lectures and seminars. During this part, relevant subjects according to the list below are treated and current articles are discussed. Especially during the seminar exercises, it is required that the students take active part in the discussions. An important aspect is to train efficient reading and extraction of information from scientific articles. A consistent theme within the course is micro- and nanostructures within biology and technology and their mutual connection. Specific subjects that are included in the course: Magnitudes in biology and physics
  • Micro- &nanofluidics: Separation and analysis of molecules and cells, soft lithography
  • Detection of single molecules: Optical, electronic and mechanical detection methods including sensor principles based on SERS, fluorescence, evanescent
  • waves.
  • Surface phenomena and surface sensors: SPR, QCM, lipid bilayer
  • The interaction of proteins and cells with nano-structured surfaces: manipulation and control of motor proteins, outgrowth of axons, antibody-antigen reactions for protein chip applications.
  • Imaging of biological structures: AFM & STM technologies.
  • Using low-dimensional electronic materials within life science.
  • System aspects: Methods for communication with the nervous system, lab-on-achip applications.

The second part consists of laboratory exercises, mainly in our research laboratories. The students will get aquainted with equipment that is actively used within the biophysics research at the division. Specific laboratory parts that are included:

  • Basic fluorescence microscopy including optical tweezers.
  • The use of total internal reflection for detection and imaging of single molecules.
  • Soft lithography and microfluidics.
  • Surface based sensors.
  • Neurobiophysics

The last part of the course is a project, where the students work individually or in small groups doing, preferably innovative but at the same time simple, experiments in a scientific environment somewhere within or outside the university. The projects are defined jointly by course responsible, supervisor and student.


Term: ​Autumn           Period: ​1​
Level: ​G​​​
Language: English
Coordinator: Jonas Tegenfeldt
Registration/Requirements: LUBAS
LTH course code​:FFFN20










Form of teaching

Lectures and seminars: Important topics are presented and discussed during lectures and in seminars.
 Laboratory exercises: Hands-on experience is gained on important biophysical tools.
Project: Focused experimental and/or theoretical study of a selected topic. Presentation of results during Minisymposium at the end of the spring semester.

 

Lectures: ​14 hours
Exercices: 20 hours





Assessment

All subparts are part of the examination. A written examination is required for grades higher than 3. A well implemented project can give additional credits on the written examination. Subcourses that are part of this course can be found in an appendix at the end of this document.​

Grading scale: U-G-VG



Literature

Course relies on articles taken from scientific literature.



Coordinator

Jonas Tegenfeldt, jonas.tegenfeldt@ftf.lth.se



phone: +46 (0)46 222 8063





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